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KMID : 0364219920350010029
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Korean Journal of Zoology
1992 Volume.35 No. 1 p.29 ~ p.36
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Identification of a Fusion-associated Protein in the Skeletal Myoblast Using Monoclonal Antibody
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Kim Chong-Rak
Choi Won-Chul
Kim Han-Do
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Abstract
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The present study describes the production of monoclonal antibodies against cultured chick myoblast to pursue critical proteins in muscle cell fusion. Among a panel of monoclonal antibodies, three, Mll-3H 13, Mll-3Hl8 and Mll-3H35 were inhibited movblast fusion. A single 101-kDa antigen reactive with monoclonal antibody Mll-3H35 was detected by radioimmu-noprecipitation or by immunoblotting. During the course of myogenesis, the level of the protein remarkably decreased as the cells there differentiated. These results suggest that the protein platys a direct role in the process of myoblast fusion mechanism.
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KEYWORD
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Myoblast, Cell fusion, Differentiation, Monoclonal antibody
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